荧光试剂柱前衍生高效液相色谱-荧光检测生物检材中的氟乙酸钠

荧光试剂柱前衍生高效液相色谱-荧光检测生物检材中的氟乙酸钠（精选2篇）

篇1：荧光试剂柱前衍生高效液相色谱-荧光检测生物检材中的氟乙酸钠

铽增敏高效液相色谱柱后衍生荧光检测法同时检测鸡肉中的三种氟喹诺酮类药物残留

基于氟喹诺酮类药物与铽离子形成配合物后的荧光增强作用,建立了同时检测鸡肉中氟喹诺酮类(FQs)药物--环丙沙星、诺氟沙星和恩诺沙星残留的`Tb3+增敏高效液相色谱(HPLC)柱后衍生荧光检测方法.优化的实验条件如下:流动相为0.05 mol/L醋酸/醋酸钠缓冲液(pH 6.0)-乙腈(体积比为89:11),色谱柱为Hypersil BDSC18,柱温40 ℃,流速0.8 mL/min;Tb3+浓度为8×10-5 mol/L;衍生反应温度40 ℃,衍生泵流速0.5 mL/min;荧光检测激发波长271 nm,发射波长545 nm.实验结果表明,将上述3种药物以1.0,10.0,50.0,100.0 ng/g水平添加到鸡肉后的回收率范围为66.3%～88.0%,相对标准偏差(RSD)均小于15.0%.定量分析的线性范围为0.1～500 n/mL,方法的日内和日间RSD均小于13.0%;最低检出限分别为0.05(环丙沙星)、0.05(诺氟沙星)和0.08(恩诺沙星)ng/g,比前人报道的非衍生高效液相色谱荧光检测法检测FQs药物的灵敏度有极大的提高.该项研究为FQs药物多残留检测提供了灵敏度更高的新方法.

作 者：祁克宗 朱良强 孙国仁 施祖灏 彭开松 QI Kezong ZHU Liangqiang SUN Guoren SHI Zuhao PENG Kaisong 作者单位：祁克宗,孙国仁,施祖灏,彭开松,QI Kezong,SUN Guoren,SHI Zuhao,PENG Kaisong(安徽农业大学动物科技学院,安徽,合肥,230036)

朱良强,ZHU Liangqiang(安徽省兽医工作站,安徽,合肥,230022)

刊 名：色谱 ISTIC PKU英文刊名：CHINESE JOURNAL OF CHROMATOGRAPHY年，卷(期)：200725(4)分类号：O658关键词：高效液相色谱 柱后衍生 氟喹诺酮类药物 多残留 铽 鸡肉

篇2：荧光试剂柱前衍生高效液相色谱-荧光检测生物检材中的氟乙酸钠

高效液相色谱-荧光检测法测定人血浆中的匹伐他汀

Pitavastatin belongs to the class of coenzyme A reductase inhibitors. Very few methods of assaying pitavastatin from human plasma are available in literature. An analytical method is presented for the determination of the drug from human plasma making use of the fluorescent property of the drug. The drug is extracted from plasma using ethyl acetate under neutral condition and then analyzed by reversed-phase high performance liquid chromatography (HPLC) with fluorescence detection (λEx 245 nm;λEm 420 nm). Analysis of pitavastatin was carried out on a C18 HPLC column using a gradient flow of mobile phase (0.01 mol/L monobasic potassium phosphate (pH 3.20)-acetonitrile, 63∶37, v/v). Fluorescein isothiocyanate was used as internal standard. The dynamic range of assay was 3 to 50 ng/mL. The intraday precision was less than 10% and accuracy ranged from 95.2% to 112.6% . The same for interday check was less than 12% and 92.8% to 105.1% , respectively. The drug was found to be stable under the assay conditions. The developed method is simple, precise, accurate, and stable. This indicates that it can be applied to routine analysis of this drug in human subjects where there are large numbers of samples without the need of specialized instruments like column switching.

作 者：OJHA Ashwini GUTTIKAR Swati VAYEDA Chintan PADH Harish 作者单位：B.V.Patel,Pharmaceutical Education & Research Development (PERD) Centre, Ahmedabad, Gujarat, India刊 名：色谱 ISTIC PKU英文刊名：CHINESE JOURNAL OF CHROMATOGRAPHY年，卷(期)：25(5)分类号：O658关键词：high performance liquid chromatography (HPLC) fluorescence detection fluorescein isothiocyanate pitavastatin human plasma